Early steps on the in vivo incorporation of 1‐14C‐linoleic acid into liver lipids from normal and essential fatty acid deficient rats

Essential fatty acid (EFA) deficient rats were injected intraportally with a solution of 1‐14C‐linoleic acid during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of 1‐14C‐linoleic acid into liver lipids...

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Bibliographic Details
Published inLipids Vol. 6; no. 11; pp. 858 - 859
Main Authors Mercuri, Osvaldo, De Tomas, Maria Elena
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer‐Verlag 01.11.1971
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Summary:Essential fatty acid (EFA) deficient rats were injected intraportally with a solution of 1‐14C‐linoleic acid during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of 1‐14C‐linoleic acid into liver lipids was measured. The results were compared with those previously obtained from normal rats. No significant differences were observed in the total radioactivity recovered from lipid extracts. While the distribution of radioactivity into the 1–2 diacylglycerol fraction remained unchanged in both groups of rats, in the EFA deficient rats the 1‐14C‐linoleic acid incorporation was actually directed to the phospholipid fractions instead of to the triacylglycerol fractions as was observed in the normal rats.
ISSN:0024-4201
1558-9307
DOI:10.1007/BF02531220