PEPSIN AS A CATALYST IN ENZYMATIC-SYNTHESIS OF PEPTIDES PARA-NITROANILIDES AND ESTERS

Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri, tetra-, penta and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe, Phe-Met, Ala-Ala-Glu, Ala-Ala-Phe, Ala-Ala-Leu, Ala-Ala-Trp, Ala-Ala-Met. Y = Ala, Leu, Val, Phe, Arg, Ala-Ala, Gly-Gly, Leu-Ala-Ala, Phe-Ala-Ala. B...

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Bibliographic Details
Published inBIOORGANICHESKAYA KHIMIYA Vol. 18; no. 8; pp. 1081 - 1088
Main Authors LYSOGORSKAYA, EN, FILIPPOVA, IY, MALAK, CAA, LAVRENOVA, GI, ANISIMOVA, VV, OKSENOIT, ES, STEPANOV, VM
Format Journal Article
LanguageRussian
Published MOSCOW Mezhdunarodnaya Kniga 01.08.1992
Subjects
Biochemistry & Molecular Biology
Chemistry
Chemistry, Organic
Life Sciences & Biomedicine
Physical Sciences
Science & Technology
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Summary:Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri, tetra-, penta and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe, Phe-Met, Ala-Ala-Glu, Ala-Ala-Phe, Ala-Ala-Leu, Ala-Ala-Trp, Ala-Ala-Met. Y = Ala, Leu, Val, Phe, Arg, Ala-Ala, Gly-Gly, Leu-Ala-Ala, Phe-Ala-Ala. B = OMe, pNA. The reactions were carried out in dimethylformamide - water solutions at pH 4.6 by equimolar ratio of amino- and carboxyl components (with the exception of Arg-pNA taken in 2-fold excess). The amount of pepsin in the reaction approached 1:1700 enzyme: substrate molar ratio although it might be improved up to 1:3.10(5) for relatively long peptides.
ISSN:0132-3423