8-1,2-Oligomannan phosphorylase-mediated synthesis of potential oligosaccharide vaccine candidates

8-(1,2)-Mannan antigens incorporated into vaccines candidates for immunization studies, showed that antibodies raised against 8-(1,2)-mannotriose antigens can protect against disseminated candidiasis. Until recently, 8-(1,2)-mannans could only be obtained by isolation from microbial cultures, or by...

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Bibliographic Details
Published inCarbohydrate research Vol. 528
Main Authors Ahmadipour, Sanaz, Winsbury, Rebecca, Kohler, Dominic, Pergolizzi, Giulia, Nepogodiev, Sergey A., Chessa, Simona, Dedola, Simone, Wang, Meng, Voglmeir, Josef, Field, Robert A.
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier 01.06.2023
Subjects
Biochemistry & Molecular Biology
Chemistry
Chemistry, Applied
Chemistry, Organic
Life Sciences & Biomedicine
Physical Sciences
Science & Technology
8 > Oligomannan phosphorylase
Glycoconjugate vaccines
Candida pathogen
Click chemistry
8 > Mannan antigen
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Summary:8-(1,2)-Mannan antigens incorporated into vaccines candidates for immunization studies, showed that antibodies raised against 8-(1,2)-mannotriose antigens can protect against disseminated candidiasis. Until recently, 8-(1,2)-mannans could only be obtained by isolation from microbial cultures, or by lengthy synthetic strategies involving protecting group manipulation. The discovery of two 8-(1,2)-mannoside phos-phorylases, Teth514_1788 and Teth514_1789, allowed efficient access to these compounds. In this work, Teth514_1788 was utilised to generate 8-(1,2)-mannan antigens, tri-and tetra-saccharides, decorated with a conjugation tether at the reducing end, suitable to be incorporated on a carrier en-route to novel vaccine can-didates, illustrated here by conjugation of the trisaccharide to BSA.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2023.108807