A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cell

Nitroreductase and acidity are common features of a tumor, which is associated with serious disease with a high mortality rate. However, solo detection of nitroreductase or acidic environments may cause false-positive results. Thus, it is very meaningful to develop dual functional probes for detecti...

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Bibliographic Details
Published inOrganic & biomolecular chemistry Vol. 16; no. 17; pp. 3266 - 3272
Main Authors He, Zhaoshuai, Chou, Yajie, Zhou, Hanxin, Zhang, Han, Cheng, Tanyu, Liu, Guohua
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 07.05.2018
Subjects
Chemistry
Chemistry, Organic
Physical Sciences
Science & Technology
OXYGEN
BIOMARKERS
FUNCTION-PH
NANOSENSORS
IN-VIVO
PURIFICATION
DOTS
HYPOXIA
CANCER
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Summary:Nitroreductase and acidity are common features of a tumor, which is associated with serious disease with a high mortality rate. However, solo detection of nitroreductase or acidic environments may cause false-positive results. Thus, it is very meaningful to develop dual functional probes for detecting nitroreductase and acidity, as these could be used for accurate tumor imaging. Here we report on the first dual functional ratiometric fluorescent probe, NAFP, for acidity and nitroreductase detection. This probe enables the measurement of the acidic microenvironment and the concentration of nitroreductase at the same time and the same site. The probe can obviously distinguish acidity, nitroreductase, and nitroreductase in an acidic environment. Furthermore, confocal fluorescence imaging of A549 cells indicates that NAFP can detect acidity and expressed nitroreductase in living cells.
ISSN:1477-0520
1477-0539
DOI:10.1039/c8ob00670a