Biogenetic studies in Syringa vulgaris L.: Bioconversion of O-18(H-2)-labeled precursors into lilac aldehydes and lilac alcohols

• Published in: Journal of agricultural and food chemistry Vol. 51; no. 25; pp. 7391 - 7395
• Main Authors: Burkhardt, D, Mosandl, A
• Format: Journal Article
• Language: English
• Published: WASHINGTON Amer Chemical Soc 03.12.2003
• Subjects: Agriculture; Agriculture, Multidisciplinary; Chemistry; Chemistry, Applied; Food Science & Technology; Life Sciences & Biomedicine; Physical Sciences; Science & Technology; stir bar sorptive extraction (SBSE); enantioselective multidimensional gas chromatography-mass spectrometry (enantio-MDGC-MS); ISOPRENOID BIOSYNTHESIS; PLANTS; 1-DEOXY-D-XYLULOSE; FLOWER; O-18(H-2)-labeled precursors; Syringa vulgaris L. biosynthesis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0304674

Syringa vulgaris L. inflorescences, petals, and chloroplasts, isolated from lilac flower petals, were fed with aqueous solutions of O-18-labeled linalool and [5,5-H-2(2)]-deoxy-D-xylose (DOX). The chloroplasts of lilac flower petals were isolated after feeding experiments with labeled precursors. Volatiles from the chloroplasts were extracted by stir bar sorptive extraction (SBSE) and analyzed using enantioselective multidimensional gas chromatography-mass spectrometry (enantio-MDGC-MS). Feeding experiments with DOX indicate that the novel mevalonate-independent 1-deoxy-D-xylose 5-phosphate/2C-methyl-D-erythritol 4-phosphate (DOX/MEP) is the decisive pathway of lilac aldehyde and lilac alcohol, respectively. Bioconversion of [O-18]linalool into lilac aldehyde and lilac alcohol during in vivo feeding experiments was monitored, and the metabolic pathways are discussed.