Evaluation of SRAP markers efficiency in genetic diversity of Aspergillus flavus from peanut-cropped soils in China

• Published in: 中国油料作物学报（英文版） Vol. 7; no. 3; pp. 135 - 141
• Main Authors: Chushu Zhang, Lifei Zhu, Mian Wang, Yueyi Tang, Haixiang Zhou, Qi Sun, Qiang Yu, Jiancheng Zhang
• Format: Journal Article
• Language: English
• Published: Shandong Peanut Research Institute,Key Laboratory of Peanut Biology and Breeding,Ministry of Agriculture and Rural Affairs,Qingdao,266100,PR China%Chongqing Normal University,Chongqing 400700,PR China%Qingdao Tianxiang Food Group Co.,Ltd,Qingdao,266737,PR China 2022
• Subjects: Peanut; Aspergillus flavus; Primer screening; Genetic diversity; Sequence-related amplified polymorphism
• ISSN: 2096-2428
• DOI: 10.3969/j.issn.2096-2428.2022.03.005

In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17 sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me1O/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.