Role of hepatitis B virus infection in pathogenesis of TgA nephropathy

• Published in: 世界胃肠病学杂志(英文版) Vol. 9; no. 9; pp. 2004 - 2008
• Main Authors: Nian-Song Wang, Zhao-Long Wu, Yue-E Zhang, Mu-Yi Guo, Lv-Tan Liao
• Format: Journal Article
• Language: English
• Published: Department of Nephrology, the Sixth Affiliated Hospital of Shanghai Jiaotong University, 600 Yushan Road,Shanghai 200233, China%Department of Nephrology,Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China%Department of Pathology, Fu Dan University, 136 Yixue Road, Shanghai 200032, China 2003
• ISSN: 1007-9327; 2219-2840

AIM: To investigate the role of hepatitis B virus (HBV) inthe pathogenesis of IgA nephropathy (IgAN).METHODS: HBV antigens (HBAg, or HBsAg, HBcAg, andHBeAg) in renal tissues with IgAN were detected byimmunohistochemical technique. The distribution andlocalization of HBV DNA were observed by using in situhybridization. Southern blot analysis was performed to revealthe state of renal HBV DNA.RESULTS: Among 100 patients with IgAN, HBs antigenemiawas detected in 18 patients (18.00%). HBAg in renal tissueswas detected in 31 patients (31.00%), the positive rate ofHBAg, HBsAg and HBcAg was 64.52%(20/31), 32.26%(10/31), 32.26% (10/31), respectively in glomeruli. HBcAgwas also found in tubular epithelia and interstitia, whichwas 45.16% (14/31) and 6.45% (2/31), respectively. Fiveout of six cases with positive HBV DNA byin situ hybridizationwere proved to be HBV DNA positive by Southern blotanalysis, and all were of the integrated form. Eight specimenswere demonstrated to be HBV DNA positive by in situhybridization, which was localized in the nuclei of tubularepithelial cells and glomerular mesangial cells as well as ininfiltrated interstitial lymphocytes.CONCLUSION: There is a relationship between HBV infectionand IgAN. In addition to the humoral immune damagemediated by HBAg-HBAb immune complex, the cellularmechanism mediated by HBV originating from renal cells insitu may be also involved in the pathogenesis of TgAN.