Cloning of gcys-18 overexpressed in Chinese gastric carcinoma and its clinicalsignificance

• Published in: 世界胃肠病学杂志(英文版) Vol. 6; no. 3; p. 29
• Main Authors: Da Xiang Cui, Xiao Jun Yan, Li Zhang, Yang Hai Guo, Jun Rong Xu, Yu Hou, Ling Xia Zhang, Cheng Zhi Su, Ning Xia Zhang
• Format: Journal Article
• Language: English
• Published: Chinese PLA Institute of Gene Diagnosis, Fourth Military Medical University, Xian 710033, Shaanxi Province, China%Department of Digestion of Xian Municipal Central Hospital, Xian 710003 2000
• Subjects: gene; stomach neoplasm; differential display; polymerase chain reaction
• ISSN: 1007-9327; 2219-2840

AIM To isolate, done and sequence gcys-18 overexpressed in gastric carcinoma.METHODS gcys-18 was isolated from differential display gel between GC7901 and GES-1 by mRNAdifferential display PCR, and was cloned into T vector. As a probe gcys-18 was hybridized to total RNAs ofGC7901 and GES-l, and was sequenced. Its sequence was screened against GeneBank. According to theobtained sequence, a pair of primers were designed and used to examine 26 specimens of gastric cancers andcorresponding paracancerous tissues by quantitative reverse transcriptase PCR.RESULTS gcys-18 was isolated and cloned, and confirmed to be expressed higher in GC7901 than in GES-1 by RNA dot blot; gcys-18 was 416bp, and partly similar to HEK5, and its accepted number in GeneBankwas AF071057; 18 out of 26 specimens of gastric cancers and 2 out of corresponding paracancerous tissueswere examined by RT-PCR.CONCLUSION gcys-18 may be an important expressed sequence tag in gastric cancer, and takes part inprogression of gastric carcinoma.