革兰氏阳性类芽孢杆菌(Paenibacillus sp.)的基因组和蛋白组研究

TQ458; 从西藏墨脱嘎隆拉山海拔3 000m土壤中分离出一株革兰氏阳性类芽孢杆菌(Paenibacillus sp.),用Illumi-na Hiseq测序平台进行全基因组测序以及通过Prokka1.14.6 软件进行基因组注释.结合antiSMASH 7.0 和BAGEL 4 分析Paenibacillus sp.基因组预测次级代谢产物生物合成基因簇.为了验证代谢产物路径,用不同破碎方法提取菌株全蛋白,通过蛋白质组质谱技术进行分析,结合不同蛋白质组分析软件,共鉴定了3 383 个蛋白质.通过同源比对,提取的蛋白质中可以检测到套索肽生物合成基因簇中依赖ATP半胱氨酸蛋白酶N端区域B1 和A...

Full description

Saved in:
Bibliographic Details
Published in应用化工 Vol. 53; no. 4; pp. 797 - 801
Main Authors 宋丽丽, 杨小琴, 张雪, 张更新, 计慕侃, 张蕾
Format Journal Article
LanguageChinese
Published 中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室,北京 100101%兰州大学 生态学院,甘肃 兰州 730000%兰州大学 泛第三极环境中心,甘肃 兰州 730000%北京工业大学科学技术发展院,北京 100101 2024
Subjects
genome
secondary metabolites
基因簇
Paenibacillus
次级代谢产物
蛋白质组
基因组
gene cluster
类芽孢杆菌
proteome
Online AccessGet full text
ISSN1671-3206
DOI10.3969/j.issn.1671-3206.2024.04.010

Cover

Abstract TQ458; 从西藏墨脱嘎隆拉山海拔3 000m土壤中分离出一株革兰氏阳性类芽孢杆菌(Paenibacillus sp.),用Illumi-na Hiseq测序平台进行全基因组测序以及通过Prokka1.14.6 软件进行基因组注释.结合antiSMASH 7.0 和BAGEL 4 分析Paenibacillus sp.基因组预测次级代谢产物生物合成基因簇.为了验证代谢产物路径,用不同破碎方法提取菌株全蛋白,通过蛋白质组质谱技术进行分析,结合不同蛋白质组分析软件,共鉴定了3 383 个蛋白质.通过同源比对,提取的蛋白质中可以检测到套索肽生物合成基因簇中依赖ATP半胱氨酸蛋白酶N端区域B1 和ABC转运器.同时,用BAGEL4 软件blastp功能从NCBI数据库中获取Paenibacillus sp.基因组中潜在完整的套索肽生物合成基因簇.
AbstractList TQ458; 从西藏墨脱嘎隆拉山海拔3 000m土壤中分离出一株革兰氏阳性类芽孢杆菌(Paenibacillus sp.),用Illumi-na Hiseq测序平台进行全基因组测序以及通过Prokka1.14.6 软件进行基因组注释.结合antiSMASH 7.0 和BAGEL 4 分析Paenibacillus sp.基因组预测次级代谢产物生物合成基因簇.为了验证代谢产物路径,用不同破碎方法提取菌株全蛋白,通过蛋白质组质谱技术进行分析,结合不同蛋白质组分析软件,共鉴定了3 383 个蛋白质.通过同源比对,提取的蛋白质中可以检测到套索肽生物合成基因簇中依赖ATP半胱氨酸蛋白酶N端区域B1 和ABC转运器.同时,用BAGEL4 软件blastp功能从NCBI数据库中获取Paenibacillus sp.基因组中潜在完整的套索肽生物合成基因簇.
Abstract_FL It isolated a Gram positive Paenibacillus sp.strain from the soil at an altitude of 3 000 m in the Kalongla Mountains in Mutuo,Tibet.The entire genome of Paenibacillus sp.strain was sequenced by Illu-mina Hiseq and was annotated with Prokka software(v1.14.6).antiSMASH 7.0 and BAGEL4 were used to predict the secondary metabolites biosynthesis gene cluster in Paenibacillus sp.In order to validate the metabolic pathway,the total strain proteins were extracted by various extraction methods and performed nanoliter liquid chromatography combining with Mass Spectrometry.We could identify a total of 3 383 pro-teins by using software.Through homologous comparison,there were the N-terminal region of ATP depend-ent Cysteine protease B1 and ABC transporter in the lasso peptide biosynthesis gene cluster.It was com-plete by local blast comparison,and Paenibacillus sp.was likely to have the ability to synthesize lasso pep-tide.Through the above research,we analyed genomic and proteomic information of Paenibacillus sp.
Author 杨小琴
张蕾
张雪
计慕侃
宋丽丽
张更新
AuthorAffiliation 中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室,北京 100101%兰州大学 生态学院,甘肃 兰州 730000%兰州大学 泛第三极环境中心,甘肃 兰州 730000%北京工业大学科学技术发展院,北京 100101
AuthorAffiliation_xml – name: 中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室,北京 100101%兰州大学 生态学院,甘肃 兰州 730000%兰州大学 泛第三极环境中心,甘肃 兰州 730000%北京工业大学科学技术发展院,北京 100101
Author_FL ZHANG Geng-xin
JI Mu-kan
YANG Xiao-qin
ZHANG Lei
SONG Li-li
ZHANG Xue
Author_FL_xml – sequence: 1
  fullname: SONG Li-li
– sequence: 2
  fullname: YANG Xiao-qin
– sequence: 3
  fullname: ZHANG Xue
– sequence: 4
  fullname: ZHANG Geng-xin
– sequence: 5
  fullname: JI Mu-kan
– sequence: 6
  fullname: ZHANG Lei
Author_xml – sequence: 1
  fullname: 宋丽丽
– sequence: 2
  fullname: 杨小琴
– sequence: 3
  fullname: 张雪
– sequence: 4
  fullname: 张更新
– sequence: 5
  fullname: 计慕侃
– sequence: 6
  fullname: 张蕾
BookMark eNrjYmDJy89LZWBQMTTQM7Y0s9TP0sssLs7TMzQzN9Q1NjIw0zMyMDLRMwAiQwMWBk64OAcDb3FxZpKBsZmJsamhqQUng__LuSuftm54tqH_5YzNzxqWP9-4-0XX3qdrFz2b2_aiv0cjIDE1LzMpMTkzJ6e0WKG4QE_z-ayWp_N3PZ294PnulqeTel7M7n4-cy-Q_XzBlOcrt_EwsKYl5hSn8kJpbgZVN9cQZw_d8sS8tMS89Pis_NKiPKBMfHFFRjrImQYmQEcaE6sOAA5wXpY
ClassificationCodes TQ458
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.3969/j.issn.1671-3206.2024.04.010
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
DocumentTitle_FL Genome and proteome analysis of Paenibacillus sp
EndPage 801
ExternalDocumentID sxhg202404010
GrantInformation_xml – fundername: (次青藏高原综合科学考察研究专项); (国家自然科学基金); (国家自然科学基金); (国家自然科学基金)
  funderid: (次青藏高原综合科学考察研究专项); (国家自然科学基金); (国家自然科学基金); (国家自然科学基金)
GroupedDBID 2B.
4A8
92I
93N
ALMA_UNASSIGNED_HOLDINGS
CDYEO
PSX
TCJ
ID FETCH-wanfang_journals_sxhg2024040103
ISSN 1671-3206
IngestDate Thu May 29 03:56:06 EDT 2025
IsPeerReviewed false
IsScholarly true
Issue 4
Keywords genome
secondary metabolites
基因簇
Paenibacillus
次级代谢产物
蛋白质组
基因组
gene cluster
类芽孢杆菌
proteome
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-wanfang_journals_sxhg2024040103
ParticipantIDs wanfang_journals_sxhg202404010
PublicationCentury 2000
PublicationDate 2024
PublicationDateYYYYMMDD 2024-01-01
PublicationDate_xml – year: 2024
  text: 2024
PublicationDecade 2020
PublicationTitle 应用化工
PublicationTitle_FL Applied Chemical Industry
PublicationYear 2024
Publisher 中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室,北京 100101%兰州大学 生态学院,甘肃 兰州 730000%兰州大学 泛第三极环境中心,甘肃 兰州 730000%北京工业大学科学技术发展院,北京 100101
Publisher_xml – name: 中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室,北京 100101%兰州大学 生态学院,甘肃 兰州 730000%兰州大学 泛第三极环境中心,甘肃 兰州 730000%北京工业大学科学技术发展院,北京 100101
SSID ssib036435158
ssib001105063
ssib000969831
ssib002258158
ssib006564880
ssib051372096
Score 4.679386
Snippet TQ458; 从西藏墨脱嘎隆拉山海拔3 000m土壤中分离出一株革兰氏阳性类芽孢杆菌(Paenibacillus sp.),用Illumi-na Hiseq测序平台进行全基因组测序以及通过Prokka1.14.6 软件进行基因组注释.结合antiSMASH 7.0 和BAGEL 4 分析Paenibacillus...
SourceID wanfang
SourceType Aggregation Database
StartPage 797
Title 革兰氏阳性类芽孢杆菌(Paenibacillus sp.)的基因组和蛋白组研究
URI https://d.wanfangdata.com.cn/periodical/sxhg202404010
Volume 53
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5CAuJFFBWfIYc0KEvivLqn-9g9O0sQfBwi5BZ2k9kkEFZxE5CcPEQPGgiIBzUEgxfJQYgIHgz5A_6PzSb_wqqa3k2vWWMUlqHpRz2-r6a3upme8bzRWqJqOZezY6om6rBAmYVSHsUwGc74s5BSyHgGDwrfuy8mHsV3p_jUwNBP56ml5aXa-MxK33Ml_8Mq1AGveEr2H5jtCoUKKAO_cAWG4XoqjlmmmCozrVjGmeTM-CwTeJUVapLMRFgjfaYTliXMBMwYlkkmNTNlHKVheIh9QI4U1FRhMoW882E1byzUqjMLi4vLzVLzCR7IQhlKMxnjUFVhRlPBMO2TeNNpClEGCIMmaWiUIoXdPgkOUUUBmoSbJKMEkFy0wlVL8i9lSlATDOGdQCEfMlISMyNJiS0cdSHvCikWHJDrMxO7UkxKXihyR_dpEdhiYiogyu6GSXi0VWr167KFpnAb8FdBB3BBahQhTwwCXghWYm3rhYYYVEhin84gxCDRCFCGlKDSlCKBfJQRgR4R74Q-9iELoT_IMZz4EIQ4jYJ6TXLAPBwOqqGSvJAhM8pCrjLSrolpqtEx3LAdqhLSoplOSwG9YpCF3AlTohFFcByG0WmhKVnS0UyKXNkPNtRTdJMUs2hp6bj4En4nAb9DflrVAqFSBJVO8WehUsR6RsYcR8gh3FSw5v_M60GtG-lWvNLHDe4XWALvbVmEa2rNhEAp-sD9r3gvin9gy0lWRBKMRaEv3GymePW2nbVjJzVJiufgO1luIej3BCpSQlEChQrGuwrG8T6iV0LbZ9B7X1HffDY_hz0gIcDTrkNhkgR80BvSpmwq7maJkj0vm_S5L9zFBJeB84wGrDTd7CKCtRN32nmAH9iiDzB27TzjjVon7pzkAh3QbNSrjTlnLTF53jtnNwFGdDGjX_AGVuYveg8ON7dbL3b2d9YP333bf_65_XX34NVe68un_c2XB-trt3rm4xGYj2-3P6y2Pv5obWy1d1dbb9YONl633-9Bub31tr39_ZLHKtlkOjFmjZi2fynN6R4go8veYONxI7_ijQhRr4pEhbIa1OMoz6uQiAEUvJrX62HO1VVv-GRZ1_7W4bp3FsvF5vINb3Dp6XJ-E5ZbS7Vhy-MvwlkI6g
linkProvider EBSCOhost
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E9%9D%A9%E5%85%B0%E6%B0%8F%E9%98%B3%E6%80%A7%E7%B1%BB%E8%8A%BD%E5%AD%A2%E6%9D%86%E8%8F%8C%28Paenibacillus+sp.%29%E7%9A%84%E5%9F%BA%E5%9B%A0%E7%BB%84%E5%92%8C%E8%9B%8B%E7%99%BD%E7%BB%84%E7%A0%94%E7%A9%B6&rft.jtitle=%E5%BA%94%E7%94%A8%E5%8C%96%E5%B7%A5&rft.au=%E5%AE%8B%E4%B8%BD%E4%B8%BD&rft.au=%E6%9D%A8%E5%B0%8F%E7%90%B4&rft.au=%E5%BC%A0%E9%9B%AA&rft.au=%E5%BC%A0%E6%9B%B4%E6%96%B0&rft.date=2024&rft.pub=%E4%B8%AD%E5%9B%BD%E7%A7%91%E5%AD%A6%E9%99%A2%E9%9D%92%E8%97%8F%E7%A0%94%E7%A9%B6%E6%89%80%E9%9D%92%E8%97%8F%E9%AB%98%E5%8E%9F%E5%9C%B0%E7%90%83%E7%B3%BB%E7%BB%9F%E4%B8%8E%E8%B5%84%E6%BA%90%E7%8E%AF%E5%A2%83%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E5%8C%97%E4%BA%AC+100101%25%E5%85%B0%E5%B7%9E%E5%A4%A7%E5%AD%A6+%E7%94%9F%E6%80%81%E5%AD%A6%E9%99%A2%2C%E7%94%98%E8%82%83+%E5%85%B0%E5%B7%9E+730000%25%E5%85%B0%E5%B7%9E%E5%A4%A7%E5%AD%A6+%E6%B3%9B%E7%AC%AC%E4%B8%89%E6%9E%81%E7%8E%AF%E5%A2%83%E4%B8%AD%E5%BF%83%2C%E7%94%98%E8%82%83+%E5%85%B0%E5%B7%9E+730000%25%E5%8C%97%E4%BA%AC%E5%B7%A5%E4%B8%9A%E5%A4%A7%E5%AD%A6%E7%A7%91%E5%AD%A6%E6%8A%80%E6%9C%AF%E5%8F%91%E5%B1%95%E9%99%A2%2C%E5%8C%97%E4%BA%AC+100101&rft.issn=1671-3206&rft.volume=53&rft.issue=4&rft.spage=797&rft.epage=801&rft_id=info:doi/10.3969%2Fj.issn.1671-3206.2024.04.010&rft.externalDocID=sxhg202404010
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fsxhg%2Fsxhg.jpg