Comparison of Lipopolysaccharide and Protein Immunogens from Pathogenic Yersinia enterocolitica Bio-serotype 1B/O:8 and 2/0:9 using SDS-PAGE

• Published in: 生物医学与环境科学（英文版） Vol. 25; no. 3; pp. 282 - 290
• Main Authors: GU Wen Peng, WANG Xin, QIU Hai Yan, LUO Xia, XIAO Yu Chun, TANG Liu Ying, KAN Biao, XU Jian Guo, JING Huai Qi
• Format: Journal Article
• Language: English
• Published: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China%National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China 2012
• Subjects: Outer membrane proteins; Whole-cell proteins; Immunogens; Lipopolysaccharides; Pathogenic Yersinia enterocolitica
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/0895-3988.2012.03.005

R378; Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system.We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y.enterocolitica.Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide (LPS),Yersinia outer membrane proteins (Yops),membrane proteins,and whole-cell proteins from poorly pathogenic Y.enterocolitica bio-serotype 2/O:9,isolated from China,and highly pathogenic bio-serotype 1B/O:8,isolated from Japan.Results These two strains of Y.enterocolitica had different LPS immune response patterns.Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity.The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains.Conclusion The major antigens of the two strains eliciting the host immune response were the LPS and membrane proteins,as shown by comparing protein samples with reference and purified preparations.