Metabolically engineered cells for the production of pinosylvin

catcatA genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia ly...

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Main Authors Katz, Michael, Förster, Jochen, David, Helga, Schmidt, Hans Peter, Sendelius, Malin, Bjørn, Sara Peterson, Durhuus, Thomas Thomasen
Format Patent
LanguageEnglish
Published 01.01.2013
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Summary:catcatA genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio K(PAL)/K(C4H) is at least 2:1.