Solid support assay systems and methods utilizing non-standard bases

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes...

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Bibliographic Details
Main Authors Grenier, Jennifer K, Marshall, David J, Prudent, James R, Richmond, Craig S, Roesch, Eric B, Scherrer, Christopher W, Sherrill, Christopher B, Ptacin, Jerod L
Format Patent
LanguageEnglish
Published 10.07.2012
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Summary:Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.