Luminescent method for determining cell oxygen consumption

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission...

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Main Authors Pitner, J. Bruce, Hemperly, John Jacob, Guarino, Richard D, Wodnicka, Magdalena, Stitt, David T, Burrell, Gregory J, Foley, Jr, Timothy G, Beaty, Patrick Shawn
Format Patent
LanguageEnglish
Published 04.03.2008
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Abstract The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
AbstractList The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
Author Foley, Jr, Timothy G
Guarino, Richard D
Burrell, Gregory J
Beaty, Patrick Shawn
Pitner, J. Bruce
Hemperly, John Jacob
Wodnicka, Magdalena
Stitt, David T
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References Berger et al. (6190612) 20010200
Di Guiseppi et al. (5164796) 19921100
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Hartmann et al. (6254829) 20010700
Bacon et al. (5030420) 19910700
Stitt et al. (5567598) 19961000
Colvin, Jr. (6711423) 20040300
Markle et al. (5511547) 19960400
Guarino et al. (6967086) 20051100
Young et al. (6063617) 20000500
Pitner et al. (6395506) 20020500
Keith (2004/0106209) 20040600
Pitner et al. (6900030) 20050500
Collins et al. (6107083) 20000800
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