Luminescent method for determining cell oxygen consumption

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission...

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Bibliographic Details
Main Authors Pitner, J. Bruce, Hemperly, John Jacob, Guarino, Richard D, Wodnicka, Magdalena, Stitt, David T, Burrell, Gregory J, Foley, Jr, Timothy G, Beaty, Patrick Shawn
Format Patent
LanguageEnglish
Published 04.03.2008
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Summary:The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.