Methods and materials for optimization of electronic transportation and hybridization reactions

Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electroph...

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Bibliographic Details
Main Authors Smolko, Daniel D, Swanson, Paul D, Hodko, Dalibor, Canter, David, Haigis, Robert W, Patterson, Tricia
Format Patent
LanguageEnglish
Published 01.01.2008
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Summary:Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.