Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase

Bacillus The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein gen...

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Main Authors Sung, Moon-Hee, Hong, Seung-Pyo, Lee, Jong-Su, Jung, Chang-Min, Hahm, Kyung-Soo, Lee, Dong-Gun, Park, Yoon Kyung, Kim, Chul-Joong, Poo, Ha-Ryoung
Format Patent
LanguageEnglish
Published 14.08.2007
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Summary:Bacillus The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.