Methods and oligonucleotides for the detection of

Salmonella E. coli Listeria monocytogenes A method for detecting a species, O157:H7, or is disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also di...

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Bibliographic Details
Main Authors Ellingson, Jay L. E, Vevea, Dirk N
Format Patent
LanguageEnglish
Published 10.07.2007
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Summary:Salmonella E. coli Listeria monocytogenes A method for detecting a species, O157:H7, or is disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also disclosed. In one embodiment, the amplification step of the method is accomplished by real-time PCR and the amplification product is detected by fluorescence resonance energy transfer using a pair of labeled polynucleotides.