Recombinant influenza A viruses

The invention is in the fields of vaccine development and application and relates to attenuated live vaccine vectors, more specifically to such vectors based on or derived from genetically modified influenza A virus strains, and to the manufacture of recominant influenza viruses and vaccines. The in...

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Main Authors Ferko, Boris, Egorov, Andre, Voglauer, Regina
Format Patent
LanguageEnglish
Published 05.10.2004
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Summary:The invention is in the fields of vaccine development and application and relates to attenuated live vaccine vectors, more specifically to such vectors based on or derived from genetically modified influenza A virus strains, and to the manufacture of recominant influenza viruses and vaccines. The invention relates to a recombinant NS gene of an influenza A virus comprising a functional RNA binding domain and a gene sequence modification after nucleotide position 400 of the NS1 gene segment, counted on the basis of influenza A/PR/8/34 Virus, wherein the modification bars transcription of the remaining portion of the NS1 gene segment. It further relates to embodiments, wherein the modification comprises deletions, insertions, or a shift of the open reading frame, and particularly to constructs comprising an insertion of an autocleavage site 2A, the nef gene from HIV-1 or the sequence encoding the ELDKWA-epitope of gp4l of HIV-1. The invention also relates to influenza virus transfectants that contain the modified NS gene and have an IFN inducing phenotype but which may or may not be sensitive towards IFN. The invention also relates to vaccines comprising such a chimeric virus.