Methods and compositions for high throughput identification of protein/nucleic acid binding pairs

• Main Authors: Gellibolian, Robert, Leproust, Eric M
• Format: Patent
• Language: English
• Published: 30.03.2004

The field of this invention is molecular biology, particularly protein/nucleic acid binding interactions and protocols for the identification thereof. Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.