Method to remove citrate and aluminum from proteins

This invention relates to a method useful for removing citrate, aluminum and other multivalent ions from biologically active proteins. The method is particularly useful for removing aluminum and citrate ions from solutions containing albumin. A method is provided for removing citrate, aluminum, and...

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Bibliographic Details
Main Authors Mamidi, Raja R, Regis, Leticia R, Mojica, Mauro C, Ito, Hirokazu, Goto, Takashi
Format Patent
LanguageEnglish
Published 17.02.2004
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Summary:This invention relates to a method useful for removing citrate, aluminum and other multivalent ions from biologically active proteins. The method is particularly useful for removing aluminum and citrate ions from solutions containing albumin. A method is provided for removing citrate, aluminum, and other multivalent ions and contaminants from proteins by adjusting the pH of a solution containing the protein to a pH from about 7 to about 10, and diafiltering the pH-adjusted solution against aqueous solutions which have a low level of ions.