Modified physiologically active proteins and medicinal compositions containing the same

• Main Authors: Takahara, Yoshiyuki, Sato, Haruya, Hayashi, Eiko, Yatagai, Masanobu, Suzuki, Manabu, Tabata, Tomoyuki, Ejima, Chieko
• Format: Patent
• Language: English
• Published: 16.09.2003

The present invention relates to a modified protein which has a liver accumulation property that its binding affinity for an asialoglycoprotein receptor present on a surface of a hepatocyte is lower than that of an asialoorosomucoid, and which can be produced by reacting a physiologically active protein containing one Gln residue that becomes a substrate for a transglutaminase with a branched-chain ligand composed of an amino acid derivative containing branched Gal or branched GalNAc in the presence of a transglutaminase to form an amide bond between the -carboxyamide group of the glutamine residue in the physiologically active protein and the terminal primary amino group in the branched-chain ligand, a process for producing the same, and a pharmaceutical composition containing the above-mentioned protein. The present invention relates to a modified physiologically active protein, modified physiologically active proteins produced by the process and pharmaceutical compositions containing the same.