Purifying microglial cells by binding cell Fc receptor to immunoglobulin G Fc domain

• Main Authors: Mi, Huaiyu, Yi, Saili
• Format: Patent
• Language: English
• Published: 01.07.2003

The invention provides methods for obtaining cell populations enriched in microglial cells. The invention finds application in the fields of medical, cell biology, and pharmacology (e.g., drug development). A method is provided for obtaining a cell population enriched in microglial cells by contacting a composition containing microglial cells with immunoglobulin immobilized on a matrix such as a polystyrene matrix before or after contact with the cells, allowing the cells to bind to the with immunoglobulin, and removing non-adherent cells to obtain a cell population containing preferably at least 95% microglial cells. The immunoglobulin may be Fdomain-containing immunoglobulin G, and Freceptors of the microglial cells bind to the Fdomain of immunoglobulin G. Purified Ffragments from immunoglobulin G may be used in place of immunoglobulin G. The microglial cells may be from brain tissue, and from tissue of a normal animal or tissue of an animal having a neurological disorder.