High throughput automatic nucleic acid isolation and quantitation methods

A high throughput RNA laboratory protocol is provided for the extraction and maintenance of a sufficient quantity of high quality RNA during sample preparation in order to analyze several genes at a time with assistance of computer analysis. The subject invention includes a method for analyzing RNA...

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Bibliographic Details
Main Authors McWilliams, Diana, Becker, Robert, Bolten, Charles, De Ciechi, Pamela, Miller, Shawn, Pinz, Andrew, Surry, Jeffrey
Format Patent
LanguageEnglish
Published 17.06.2004
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Summary:A high throughput RNA laboratory protocol is provided for the extraction and maintenance of a sufficient quantity of high quality RNA during sample preparation in order to analyze several genes at a time with assistance of computer analysis. The subject invention includes a method for analyzing RNA comprising the steps of extracting RNA from a complex biological construct in sufficient quantities to provide accurate RNA data, transferring RNA to an apparatus that maintains the RNA and necessary reagents at a temperature of between about 0 to 10° C., and analyzing RNA with a computer generated mathematical analysis of the data to access the presence of RNA and ultimately test the efficacy of a drug.