Immunoassays and Characterization of Biomolecular Interactions Using Self-Assembled Monolayers

• Format: Patent
• Language: English
• Published: 23.09.2009

The present disclosure relates to methods of characterizing protein-protein interaction or conducting immunoassays in a biological sample using self-assembled monolayers (SAMs) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (SAMDI). The biological sample may be obtained from a living subject, such as a human or animal clinical sample containing multiple unknown proteins and/or antigens. Label-free methods of identifying protein-protein interactions, antigen-antibody binding and/or diagnosing a medical condition based on analysis of a biological sample using SAMDI are also provided, as well as biochips comprising surface bound proteins and/or antibodies and methods of making these biochips. The methods and biochips are useful, for example, for identifying protein-protein binding interactions and/or conducting immunoassays in samples such as humoral fluids or other clinical samples, cell lysates, tissue lysates, tumor lysates, and samples obtained, isolated or derived from animals or plants.