Use of Andromed® and OviXcell® diluents in the processing of sheep semen with the addition of HTF

• Published in: Agro Productividad Vol. 16; no. 12 (supplemental); pp. 41 - 48
• Main Authors: Quintero-Elisea, Juan A, Clemente-Sánchez, Fernando, Velázquez-Morales, José V, Vázquez-Rocha, Lizeth, Quintanilla-Medina Jairo J
• Format: Journal Article
• Language: English
• Published: 31.01.2024
• Edition: 2394
• Subjects: Andromed; Diluents; HTF; Livestock Production/Industries; OviXcell; sheep
• ISSN: 2594-0252
• DOI: 10.22004/ag.econ.347834

Objective: To assess the effect of two ovine semen diluents, Andromed® and OviXcell®, on the mass motility, vitality, and acrosomal integrity of spermatozoa processed at different temperatures and with the addition of post-thawed HTF (human tubal fluid). Design/Methodology/Approach: Semen from 2 Dorper ram was used. Four ejaculations per male were collected and diluted with Andromed® and Ovixcell®. There were four treatments with Ovixcell®: 1) fresh Ovixcell®, 2) refrigerated Ovixcell®, 3) post-thawed Ovixcell®, and 4) post-thawed Ovixcell®  HTF. Four treaments with Andromed® were also carried out: 1) fresh Andromed®, 2) refrigerated Andromed®, 3) postthawed Andromed®, and 4) post-thawed Andromed®  HTF. Mass motility, vitality, and acrosomal integrity were performed with a CASA computer system and statistically analyzed with the GLM procedure of the SAS software. Results: When the effect of Ovixcell® mass motility was assessed, the following results were obtained, with differences (p0.05) between treatments: 87% in fresh semen, 72% refrigerated semen, 55% in post-thawed semen, and 68% in post-thawed semen  HTF. A clear difference (p0.05) was observed when HTF was added to post-thawed semen (13%). Andromed® behaved in the same way as Ovixcell® (p0.05) and a 18% recovery was observed with the addition of HTF. A high percentage of live spermatozoa with intact acrosome was observed for fresh semen (97.8%), while it diminished (p0.05) as the temperature of refrigerated and frozen semen gradually decreased. Study Limitations/Implications: More experimental units should be used, despite the increase in maintenance costs per animal. Findings/Conclusions: The use of Andromed® and OviXcell® diluents was satisfactory in relation to the mass motility, vitality, and acrosomal integrity in spermatozoa processed at different temperatures (fresh, refrigerated, and post-thawed). Furthermore, the addition of HTF increased post-thawed mass motility.