Reverse Phase Protein Array (RPPA) of High Risk ALL

• Published in: Klinische Pädiatrie
• Main Authors: Seyfried, F, Accordi, B, Queudeville, M, Eckhoff, SM, Milani, G, Galla, L, Giordan, M, Kraus, J, Basso, G, Kestler, H, te Kronnie, G, Debatin, KM, Meyer, LH
• Format: Conference Proceeding
• Language: English
• Published: 27.04.2012
• ISSN: 0300-8630; 1439-3824
• DOI: 10.1055/s-0032-1310475

In a NOD/SCID/huALL model we have recently shown that short time to leukemia (TTL short ) of xenografted patient samples determines poor patient outcome. In this study we analyzed 16 xenografted BCP-ALL samples using RPPA and validated the findings by Western Blot. Comparison of the protein expression data in short vs. long TTL subgroups (shrinkage t-test, P<0.05, fold change ≥1.5) revealed up-regulation of CYCLIN B, ß-CATENIN and ANNEXIN I and down-regulated PKCd in TTL short ALL. Consistently, CYCLIN B is a positive regulator of the cell cycle suggesting an association of cell cycle progression and NOD/SCID engraftment. ß-CATENIN is involved in WNT-signaling and associated with apoptosis inhibition and cell growth. ANNEXIN I is a regulator of inflammation and reported to be over-expressed in hairy cell leukemia. In contrast, down-regulated PKCd has also been reported in poor prognostic T-ALL patients. Taken together, this study identified differenzially expressed proteins in prognostic subgroups of BCP-ALL patients with distinct clinical outcomes, which can be further evaluated as new prognostic markers and therapeutic targets.