Reverse Phase Protein Array (RPPA) of High Risk ALL
In a NOD/SCID/huALL model we have recently shown that short time to leukemia (TTL short ) of xenografted patient samples determines poor patient outcome. In this study we analyzed 16 xenografted BCP-ALL samples using RPPA and validated the findings by Western Blot. Comparison of the protein expressi...
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Published in | Klinische Pädiatrie |
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Main Authors | , , , , , , , , , , , , |
Format | Conference Proceeding |
Language | English |
Published |
27.04.2012
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Online Access | Get full text |
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Summary: | In a NOD/SCID/huALL model we have recently shown that short time to leukemia (TTL
short
) of xenografted patient samples determines poor patient outcome. In this study we analyzed 16 xenografted BCP-ALL samples using RPPA and validated the findings by Western Blot.
Comparison of the protein expression data in short vs. long TTL subgroups (shrinkage t-test, P<0.05, fold change ≥1.5) revealed up-regulation of CYCLIN B, ß-CATENIN and ANNEXIN I and down-regulated PKCd in TTL
short
ALL. Consistently, CYCLIN B is a positive regulator of the cell cycle suggesting an association of cell cycle progression and NOD/SCID engraftment. ß-CATENIN is involved in WNT-signaling and associated with apoptosis inhibition and cell growth. ANNEXIN I is a regulator of inflammation and reported to be over-expressed in hairy cell leukemia. In contrast, down-regulated PKCd has also been reported in poor prognostic T-ALL patients.
Taken together, this study identified differenzially expressed proteins in prognostic subgroups of BCP-ALL patients with distinct clinical outcomes, which can be further evaluated as new prognostic markers and therapeutic targets. |
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ISSN: | 0300-8630 1439-3824 |
DOI: | 10.1055/s-0032-1310475 |