Genetic diversity of marine oligochaetous clitellates in selected areas of the Southern Atlantic as revealed by DNA barcoding

• Published in: Invertebrate systematics Vol. 32; no. 3; p. 524
• Main Authors: Prantoni, Alessandro L, Belmonte-Lopes, Ricardo, Lana, Paulo C, Erséus, Christer
• Format: Journal Article
• Language: English
• Published: 2018
• Subjects: Biological Systematics; Biologisk systematik; COI; DNA barcodes; marine clitellates; southern Atlantic Ocean; species delimitation; Zoologi; Zoology
• ISSN: 1445-5226; 1447-2600
• DOI: 10.1071/IS17029

Marine oligochaetous clitellates are poorly investigated in the South Atlantic Ocean, especially along the east coast of South America. Closely related species are often difficult to distinguish based on morphology. The lack of specialists and modern identification guides have been pointed out as the main reasons for the scarcity of studies in the South Atlantic Ocean as a whole. To increase the knowledge of this group in the South Atlantic, the genetic diversity of a sample of marine oligochaetous clitellates from Brazil, South Africa and Antarctica was assessed by the Automatic Barcode Gap Discovery (ABGD) and the generalised mixed Yule coalescent (GMYC) approaches. In total, 80 cytochrome c oxidase subunit I (COI) sequences were obtained, each with ~658 bp, estimated to represent 32 distinct putative species. ABGD established a barcoding gap between 3% and 14% divergence for uncorrected p-distances and the estimates of GMYC were largely concordant. All the clusters or putative species were genetically associated with previously known species or genera. This study thus confirms the adequacy of the COI barcoding approach combined with a genetic divergence threshold at the order of 10% for marine oligochaetous clitellates.