Development of the Tracer-Pulse method for adsorption studies of analyte mixures in liquid chromatography utilizing mass spectrometric detection

The tracer-pulse method provides the real adsorption data points directly from simple, straightforward calculations and is therefore a superior method for multicomponent adsorption isotherm determination in HPLC. Only one important problem has restricted its use so far: the tracer peaks are invisibl...

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Published inAnalytical chemistry (Washington) Vol. 80; no. 6; p. 2105
Main Authors Samuelsson, Jörgen, Arnell, Robert, Diesen, Jarle, Tibbelin, Julius, Paptchikhine, Alexander, Fornstedt, Torgny, Sjöberg, Per J. R.
Format Journal Article
LanguageEnglish
Published 2008
Subjects
Chemistry
Kemi
NATURAL SCIENCES
NATURVETENSKAP
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Summary:The tracer-pulse method provides the real adsorption data points directly from simple, straightforward calculations and is therefore a superior method for multicomponent adsorption isotherm determination in HPLC. Only one important problem has restricted its use so far: the tracer peaks are invisible using any conventional detection principle. We present a solution to this problem with an approach with a firm base in analytical chemistry, utilizing stable isotopes and mass spectrometric detection. The new approach was used for the determination of binary adsorption isotherms, and a systematic investigation was made of its main sources of error. With this modification, the tracer method can be a prime choice for future characterizations of multicomponent separation systems and of competitive drug binding studies.
ISSN:1520-6882
0003-2700
DOI:10.1021/ac702399a