RETRACTED ARTICLE: Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance

Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a t...

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Bibliographic Details
Published inMolecules and cells Vol. 34; no. 1; pp. 43 - 52
Main Authors Huh, Sung Un, Lee, In-Ju, Ham, Byung-Kook, Paek, Kyung-Hee
Format Journal Article
LanguageEnglish
Published Springer Korean Society for Molecular and Cellular Biology 2012
Subjects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Cell Biology
Life Sciences
NtTfd1
VIGS
Tsip1
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Summary:Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo . The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1 , and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1 - and Tsip1 -silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.
ISSN:1016-8478
0219-1032
DOI:10.1007/s10059-012-0066-6