Nucleopolyhedrovirus: scanning electron microscopy technique

A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovi...

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Published inNeotropical entomology Vol. 35; no. 6; pp. 787 - 790
Main Authors Torquato, Ednéia F.B., Miranda Neto, Marcílio H. de, Brancalhão, Rose M.C., Franco, Valdeni S.
Format Journal Article
LanguagePortuguese
Published Sociedade Entomológica do Brasil 01.12.2006
Subjects
ENTOMOLOGY
poliedro
Baculovírus
BmMNPV
octaedro truncado
Bombyx mori
Baculovirus
polyhedra
truncated octahedron
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Summary:A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identified it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specific for each viral lineage.
ISSN:1678-8052
DOI:10.1590/S1519-566X2006000600011