Characterization of Cuban native bacteria isolated from nematodes as potential biological control agents for Meloidogyne spp

The objectives of this study were the molecular and conventional characterization of three native strains isolated from parasitic nematodes, evaluation of their potential to control Meloidogyne spp., and determination of their possible pathogenicity traits against nematodes. The identity of the stra...

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Published inRevista de protección vegetal
Main Authors Sánchez Ortiz, Ileana, Alvarez Lugo, Irene, Wong Padilla, Idania, Somontes, Danalay, Basulto Morales, Rosa, Morán Valdivia, Rolando, Pimentel Vázquez, Eulogio, Mena Campos, Jesús
Format Journal Article
LanguagePortuguese
Published Centro Nacional de Sanidad Agropecuaria 01.04.2018
Subjects
ACOUSTICS
Stenotrophomonas
Bacillus
root knot nematodes
control biológico
Stenotrophomonasmaltophilia
biological control
Sphingobacterium
nematodos agalleros
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Summary:The objectives of this study were the molecular and conventional characterization of three native strains isolated from parasitic nematodes, evaluation of their potential to control Meloidogyne spp., and determination of their possible pathogenicity traits against nematodes. The identity of the strains Bacillus thuringiensis CIGBR23, Sphingobacterium sp. CIGBTb, and Stenotrophomonasmaltophilia CIGBG1 was confirmed by 16S rRNA sequencing. Their effectiveness to reduce infestation of Meloidogyne spp. was evaluated in Cucurbita maxima RG-T150 as host plant growing in pot. The plant growth-promoting effects of the strains were also determined. The root-galling index, branch length, and branch and root fresh weights were determined 35 days after nematode inoculation. The three strains controlled Meloidogyne spp. The treatment with Sphingobacterium sp.CIGBTbwasthe most effective, reducing the infestation index from 3 to 1; whereas it was 1.6 for B. thuringiensis CIGBR23 and 1.7 for S.maltophilia CIGBG1 (Hussey and Janssen’s scale 0 -5). Sphingobacterium sp.CIGBTb and S.maltophilia CIGBG1 also significantly reduced the number of root galls by C. maxima (higher than 50 %)in relation to the control (p˂0.05). On the other hand, the treatment with B. thuringiensis CIGBR23 increased plant weight in 17 %. The three strains produced chitinase enzymes. Two of the strains (CIGBR23 and CIGBG1) also excreted lipases and proteases, and CIGBG1, in addition to these enzymes, also produced phospholipase and hydrogen sulfide
ISSN:2224-4697
2224-4697