Comparison of preservation methods for the maintenance of brewing yeast : research in action

Preservation methods (cryopreservation at -196oC in liquid nitrogen, freezing at -70oC, and lyophilization) were compared for the maintenance of brewing inocula over a period of two years. A decrease in the percentage of variants and respiratory deficient yeasts was generally found when test materia...

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Bibliographic Details
Published inSouth African journal of science Vol. 98; no. 1; pp. 23 - 24
Main Authors Morakile, G., Pretorius, E.E., Pohl, C.H., Kock, J.L.F.
Format Journal Article
LanguageEnglish
Published Academy of Science for South Africa (ASSAf) 01.01.2002
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Summary:Preservation methods (cryopreservation at -196oC in liquid nitrogen, freezing at -70oC, and lyophilization) were compared for the maintenance of brewing inocula over a period of two years. A decrease in the percentage of variants and respiratory deficient yeasts was generally found when test material was preserved by cryopreservation and freezing. By contrast, the percentage of variants when revived yeasts were grown in wort increased when maintained by lyophilization. A high percentage (>95%) of viable cells was recorded for yeast cultures maintained at -196oC and at -70oC, whereas viability was low (<50%) when maintained by lyophilization. The maintenance methods of choice are therefore cryopreservation and freezing.
ISSN:0038-2353
1996-7489