Smartphone-based sensing and and imaging of Mn() based on nitrogen-doped red fluorescent carbon dots
Smartphone-assisted visual assay platform provides novel insight for the real-time in-field quantitation of intended analytes in resource-insufficient areas. Herein, nitrogen-doped red fluorescent (FL) carbon dots (R-CDs) were developed for the timely on-site quantitation of Mn( vii ) using the smar...
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Published in | Analytical methods Vol. 16; no. 23; pp. 3757 - 3765 |
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Main Authors | , , , |
Format | Journal Article |
Published |
13.06.2024
|
Online Access | Get full text |
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Summary: | Smartphone-assisted visual assay platform provides novel insight for the real-time in-field quantitation of intended analytes in resource-insufficient areas. Herein, nitrogen-doped red fluorescent (FL) carbon dots (R-CDs) were developed for the timely on-site quantitation of Mn(
vii
) using the smartphone-assisted assay platform. R-CDs, possessing a desirable bright red FL at 616 nm under a 470 nm excitation, were fabricated through hydrothermal treatment adopting passion fruit and neutral red as precursors. Interestingly, bright red FL at 616 nm are gradually quenched upon introducing Mn(
vii
) based on the inner filter effect, concurrently accompanying with significant FL color variation from bright red to dark red. Inspired by the above-mentioned phenomena, hue-saturation-values (HSV) of real-time captured images could be precisely quantified through a color recognition APP within the smartphone, of which the V/S values could be employed to quantify Mn(
vii
) with a linear range of 50-400 μM. Furthermore, confocal fluorescence imaging of HeLa cells and zebrafish larvae demonstrates that R-CDs could be employed for the visual determination of Mn(
vii
)
in vivo
and
in vitro
, illustrating that R-CDs possess powerful practical application prospect in biosystem.
Schematic diagram of synthesis and application of R-CDs for visual quantification and bioimaging of Mn(
vii
). |
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Bibliography: | Electronic supplementary information (ESI) available. See DOI https://doi.org/10.1039/d4ay00783b |
ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/d4ay00783b |