Bio-guided isolation of alpha-glucosidase inhibitory compounds from Vietnamese fruits: and studies
Garcinia schomburgkiana is an edible tree widely distributed in the southern region of Vietnam. Little is known about the alpha-glucosidase inhibition of the Vietnamese Garcinia schomburgkiana . The aim of the current study was to explore the anti-diabetic potential of G. schomburgkiana fruits. All...
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| Published in | RSC advances Vol. 13; no. 5; pp. 3548 - 35421 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Published |
04.12.2023
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| Online Access | Get full text |
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| Summary: | Garcinia schomburgkiana
is an edible tree widely distributed in the southern region of Vietnam. Little is known about the alpha-glucosidase inhibition of the Vietnamese
Garcinia schomburgkiana
. The aim of the current study was to explore the anti-diabetic potential of
G. schomburgkiana
fruits. All the fractions of
G. schomburgkiana
were evaluated for alpha-glucosidase inhibition, followed by bioassay-guided isolation. A new compound,
epi
-guttiferone Q (
1
), together with ten known compounds, guttiferones I-K (
2-3
), hypersampsone I (
4
), sampsonione D (
5
), sampsonione H (
6
), β-mangostin (
7
), α-mangostin (
8
), 9-hydroxycalabaxanthone (
9
), and fuscaxanthone (
10
), were isolated and structurally elucidated. The structure of the new metabolite
1
was confirmed through 1D and 2D NMR spectroscopy, and MS analysis. To the best of our knowledge, the metabolites (except
3
) have not been isolated from this plant previously. All isolated compounds were evaluated for their alpha-glucosidase inhibition. Compounds
1-6
showed potent activity with IC
50
values ranging from 16.2 to 130.6 μM. Compound
2
was further selected for a kinetic study. The result indicated that it was a competitive type. Additionally,
in silico
docking was employed to predict the binding mechanism of
1-2
and
4-6
in the active site of alpha-glucosidase, suggesting their potential as promising anti-diabetic compounds. Molecular dynamic simulation was also applied to
1
to better understand its inhibitory mechanism.
Garcinia schomburgkiana
is an edible tree widely distributed in the southern region of Vietnam. |
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| Bibliography: | https://doi.org/10.1039/d3ra06760b Electronic supplementary information (ESI) available. See DOI |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/d3ra06760b |