Stable biogenic silver nanoparticles from bud extract for enhanced catalytic, antibacterial and antifungal properties

• Published in: RSC advances Vol. 13; no. 3; pp. 2994 - 217
• Main Authors: Lan Pham, Thi, Dat Doan, Van, Le Dang, Quang, Anh Nguyen, Tuan, Huong Nguyen, Thi Lan, Thuy Tran, Thi Dieu, Lan Nguyen, Thi Phuong, Anh Vo, Thi Kieu, Huy Nguyen, Trung, Lam Tran, Dai
• Format: Journal Article
• Published: 12.07.2023
• ISSN: 2046-2069
• DOI: 10.1039/d3ra02754f

In the present study, the biosynthesis of stable silver nanoparticles (BioAgNPs) was accomplished successfully for the first time by using an aqueous extract derived from the buds of Syzygium nervosum (SN) as both a reducing and a stabilizing agent. Transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM) investigations revealed that the biosynthesized BioAgNPs were predominantly spherical with an average size of 10-30 nm. It was found that the outstanding stability of the BioAgNPs colloidal solution was assigned to the additive effect of the surrounding protective organic layer and the highly negatively charged surface of the nanoparticles. Consequently, good antibacterial activity was demonstrated by the colloidal BioAgNPs solution against four distinct bacterial strains, including Gram-positive S. aureus and B. subtilis as well as Gram-negative E. coli and S. typhi . Interestingly, the biosynthesized BioAgNPs displayed greater antibacterial activity even when tested at low doses against Gram-negative S. typhi . In addition, the biogenic AgNPs demonstrated a significant level of catalytic activity in the process of converting 2-NP, 3-NP, and 4-NP into aminophenols within 15 min, with reaction rate constants of 9.0 × 10 −4 , 10 × 10 −4 , and 9.0 × 10 −4 s −1 , respectively. BioAgNPs formulations were assessed against anthracnose disease in tea plants and were found to be as effective as the positive control at a dose of 20-fold dilution, but less effective at a dose of 30-fold dilution. Both doses of BioAgNPs formulations significantly suppressed Colletotrichum camelliae (anthracnose disease) without affecting the growth of the tea plants. Stable BioAgNPs were synthesized utilizing Syzygium nervosum bud extract without using any additional commercial chemicals. The BioAgNPs exhibit promising potential as a catalyst for nitrophenol treatment and as an effective agent for disease treatment and crop protection.