Self-decorating cells surface-initiated enzymatic controlled radical polymerization

Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting...

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Bibliographic Details
Published inNanoscale Vol. 15; no. 48; pp. 19486 - 19492
Main Authors Belluati, Andrea, Happel, Dominic, Erbe, Malte, Kirchner, Nicole, Szelwicka, Anna, Bloch, Adrian, Berner, Valeria, Christmann, Andreas, Hertel, Brigitte, Pardehkhorram, Raheleh, Reyhani, Amin, Kolmar, Harald, Bruns, Nico
Format Journal Article
Published 14.12.2023
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Summary:Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering. Through the use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT and bioATRP to prompt polymer synthesis on the surface of S. cerevisiae cells, with bioATRP outperforming bioRAFT polymerization.
Bibliography:https://doi.org/10.1039/d3nr04008a
Electronic supplementary information (ESI) available. See DOI
ISSN:2040-3364
2040-3372
DOI:10.1039/d3nr04008a