HF01 fermented yogurt alleviated high-fat diet-induced obesity and hepatic steatosis the gut microbiota-butyric acid-hepatic lipid metabolism axis

• Published in: Food & function Vol. 15; no. 8; pp. 4475 - 4489
• Main Authors: Sun, Yue, Liu, Xiaolin, Wang, Rongchun, Liu, Rongmei, Lv, Xuepeng, Ma, Ying, Li, Qiming
• Format: Journal Article
• Published: 22.04.2024
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo04985j

The objective of this study was to investigate the anti-obesity effects and underlying mechanism of Lacticaseibacillus rhamnosus HF01 fermented yogurt (HF01-Y). Herein, obesity was induced in mice through a high-fat diet and the changes in the gut microbiota were evaluated using 16S rRNA gene sequencing, combined with the expression levels of the liver AMPK signaling pathway to analyze the potential relationship between HF01-Y-mediated gut microbiota and obesity. The results showed that supplementation with HF01-Y improved obesity-related phenotypes in mice, including reduced body weight, improved serum lipid profiles, and decreased hepatic lipid droplet formation. In addition, HF01-Y altered the composition of the gut microbiota in obese mice, significantly upregulated norank_f__Muribaculaceae , unclassified_c__Clostridia , Blautia , unclassified_o__Bacteroidales , and Rikenellaceae_RC9_gut_group , while downregulating unclassified_f__Desulfovibrionaceae , Colidextribacter , and unclassified_f__Oscillospiraceae . These alterations led to an increase of the cecum butyric acid content, which in turn indirectly promoted the activation of the AMPK signaling pathway, subsequently, inhibited fat synthesis, and promoted fatty acid oxidation related gene expression. Therefore, HF01-Y was likely to alleviate hepatic fat and relieve obesity by modulating the gut microbiota-butyric acid-hepatic lipid metabolism axis, ultimately promoting host health. The fermented yogurt of Lacticaseibacillus rhamnosus HF01 alters obese mice gut microbiota by boosting beneficial flora and butyric acid, activates AMPK to regulate lipid metabolism, combating obesity.