AgPt/MoS hybrid as electrochemical sensor for detecting HO release from living cells

• Published in: New journal of chemistry Vol. 46; no. 31; pp. 1532 - 1541
• Main Authors: Zhu, Xiaona, Wang, Zegao, Gao, Mingyan, Wang, Yuqing, Hu, Jing, Song, Zhengxun, Wang, Zuobin, Dong, Mingdong
• Format: Journal Article
• Published: 08.08.2022
• ISSN: 1144-0546; 1369-9261
• DOI: 10.1039/d2nj02495k

Hydrogen peroxide (H 2 O 2 ), as a biomarker in a series of physiological processes, plays a crucial role in investigating the pathogenesis of some diseases. The highly sensitive and selective detection of H 2 O 2 release from living cells has aroused tremendous research interests. Herein, a non-enzymatic electrochemical H 2 O 2 biosensor was constructed based on AgPt bimetallic nanoparticle-structured MoS 2 nanohybrid (AgPt/MoS 2 ) as an electrocatalyst. The AgPt/MoS 2 nanohybrid was successfully synthesized by a facile wet-chemical method. Compared with AgPt nanoparticles, the AgPt/MoS 2 nanohybrid exhibited significant enhancement in catalytic performance towards H 2 O 2 . The main factor is that MoS 2 nanosheets as a supporting material promote AgPt nanoparticles to expose more active sites. The electrochemical response of the AgPt/MoS 2 hybrid-based biosensor to H 2 O 2 was linear in the range of 20 μM-4 mM, and the detection limit was calculated as 1.0 μM. To prove practicality, the biosensor was further applied to detect H 2 O 2 release from living SMMC-7721 cells. These results indicate the potential applications of the developed biosensor in monitoring physiological processes. A novel non-enzymatic H 2 O 2 biosensor based on a AgPt/MoS 2 nanohybrid exhibits high sensitivity and selectivity.