Catalase-albumin cluster incorporating protoporphyrin IX: an O generating photosensitizer for enhanced photodynamic therapy
Photodynamic therapy (PDT) is a non-invasive cancer treatment using reactive oxygen species (ROS) generated by light irradiation. An excited state photosensitizer produces active radicals ( e.g. O 2 &z.rad; − and OH&z.rad;) by electron transfer reactions (type I), while an excited triplet st...
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| Published in | Materials advances Vol. 3; no. 16; pp. 6451 - 6457 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Published |
15.08.2022
|
| Online Access | Get full text |
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| Summary: | Photodynamic therapy (PDT) is a non-invasive cancer treatment using reactive oxygen species (ROS) generated by light irradiation. An excited state photosensitizer produces active radicals (
e.g.
O
2
&z.rad;
−
and OH&z.rad;) by electron transfer reactions (type I), while an excited triplet state photosensitizer generates singlet O
2
(
1
O
2
) by direct energy transfer to molecular O
2
(type II). This report describes the synthesis and PDT activity of the hemoprotein-based photosensitizer combined with O
2
production capability. Covalent wrapping of a catalase (Cat) with human serum albumins (HSAs) yielded the core-shell structured protein cluster Cat-HSA
5
, in which an average of five HSA molecules are bound to the Cat center. Cat-HSA
5
retained the high enzymatic activity of the hydrogen peroxide (H
2
O
2
) disproportionation reaction. Subsequently, the incorporation of protoporphyrin IX (PP) into an individual HSA unit resulted in a Cat-(HSA-PP)
5
cluster. The
1
O
2
generation ability of Cat-(HSA-PP)
5
was equivalent to that of the HSA-PP complex. Cytotoxicity with light irradiation was evaluated using HeLa cells. Cat-(HSA-PP)
5
demonstrated higher PDT activity than the HSA-PP complex. The Cat core provoked conversion of endogenous H
2
O
2
to O
2
efficiently in the cells to facilitate
1
O
2
formation in large amounts. Cat-(HSA-PP)
5
is a unique dual functional hemoprotein cluster (O
2
generation and photosensitization) that can achieve enhanced PDT.
Catalase enwrapped covalently with human serum albumin incorporating protoporphyrin IX is a unique dual functional hemoprotein cluster (O
2
generation and photosensitization) that can achieve enhanced photodynamic therapy. |
|---|---|
| Bibliography: | and cell viability of HeLa cells treated with Cat-(HSA-PP) generated by H time course of O Electronic supplementary information (ESI) available: Native-PAGE, SDS-PAGE and DLS of Cat-HSA https://doi.org/10.1039/d2ma00242f O degradation using Cat-HSA 2 absorption and fluorescence spectra of HSA-PP, fluorescence, transient absorption spectra and time course of the fluorescence intensity of Cat-(HSA-PP) without or with light irradiation. See DOI 5 Lineweaver-Burk plots of H dismutation using Cat-HSA |
| ISSN: | 2633-5409 |
| DOI: | 10.1039/d2ma00242f |