Enhanced enantioseparation of drugs by capillary electrochromatography with a -cysteine functionalized gold nanoparticle based stationary phase

• Published in: Analytical methods Vol. 14; no. 2; pp. 1982 - 1987
• Main Authors: Miao, Pandeng, Xi, Ying, Feng, Zijie, Zhang, Jian, Du, Yingxiang, Chen, Cheng
• Format: Journal Article
• Published: 27.05.2022
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/d2ay00414c

Nanoparticles, which have unique properties, have attracted growing attention in enantiomeric separation nowadays. In this paper, an l -cysteine functionalized gold nanoparticle ( l -Cys-GNP) based capillary column was prepared and applied in separating drug enantiomers in capillary electrochromatography (CEC) with lactobionic acid (LA) as a chiral selector. Compared with bare fused-silica capillary columns, the capillary columns modified with l -Cys-GNPs showed excellent chiral separation performance. A series of parameters affecting the enantiomeric separation were systematically investigated. l -cysteine-functionalized gold nanoparticles have significantly improved the enantioseparation performance of capillary electrochromatography with reliable stability and repeatability.