A phenanthrene[9,10-]imidazole-phenol-based fluorescent probe combining ESIPT and AIE for the "turn-on" detection of Cu with green-emission and improved Stokes' shift, and its application
The development of highly sensitive and selective fluorescent sensors toward Cu 2+ has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by the fluorescence "turn-on" mode at a longer wavelength has not been we...
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Published in | New journal of chemistry Vol. 45; no. 34; pp. 15415 - 15422 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Published |
31.08.2021
|
Online Access | Get full text |
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Summary: | The development of highly sensitive and selective fluorescent sensors toward Cu
2+
has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by the fluorescence "turn-on" mode at a longer wavelength has not been well explored until now. In the present study, we report a novel green-emitting Cu
2+
-targeted fluorescent sensor,
PIA(OH)-Py
, with an improved Stokes' shift (182 nm), synergistically operated by the multi-mechanism based on the excited state intramolecular proton transfer (ESIPT) process combined with the aggregation-induced emission (AIE) phenomenon. A moderate fluorescence of
PIA(OH)-Py
was first fabricated with the effect of ESIPT-on as well as AIE-on. In the presence of Cu
2+
, the strong emission of the
in situ
complex
PIA(OH)-Py
-Cu
2+
was obtained by the impact of further AIE-on accompanied by ESIPT-off as a fluorescence "turn on" response. In addition, the chelation-enhanced fluorescence (CHEF) mechanism was also partly responsible for the fluorescence increment due to the formation of the complex
PIA(OH)-Py
-Cu
2+
. Further study revealed that
PIA(OH)-Py
could respond to Cu
2+
in living cells by the green light channel and determine the concentration of Cu
2+
in real blood samples.
The "turn-on" probe
PIA(OH)-Py
responds to Cu
2+
in living cells and can determine the concentration of Cu
2+
in blood samples. |
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Bibliography: | 10.1039/d1nj02177j Electronic supplementary information (ESI) available. See DOI |
ISSN: | 1144-0546 1369-9261 |
DOI: | 10.1039/d1nj02177j |