Gd-based site-directed spin labeling and EPR spectroscopy of eGFP

• Published in: Physical chemistry chemical physics : PCCP Vol. 22; no. 24; pp. 13358 - 13362
• Main Authors: Kucher, Svetlana, Korneev, Sergej, Klare, Johann P, Klose, Daniel, Steinhoff, Heinz-Jürgen
• Format: Journal Article
• Language: English
• Published: 24.06.2020

Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd 3+ -DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscop...