Gd-based site-directed spin labeling and EPR spectroscopy of eGFP

Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd 3+ -DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscop...

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Published inPhysical chemistry chemical physics : PCCP Vol. 22; no. 24; pp. 13358 - 13362
Main Authors Kucher, Svetlana, Korneev, Sergej, Klare, Johann P, Klose, Daniel, Steinhoff, Heinz-Jürgen
Format Journal Article
LanguageEnglish
Published 24.06.2020
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Summary:Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd 3+ -DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures. A newly synthesized Gd 3+ chelate complex allows in cell spin labeling and detection of eGFP by EPR spectroscopy.
Bibliography:10.1039/d0cp01930e
Electronic supplementary information (ESI) available. See DOI
ISSN:1463-9076
1463-9084
DOI:10.1039/d0cp01930e