Gd-based site-directed spin labeling and EPR spectroscopy of eGFP
Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd 3+ -DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscop...
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Published in | Physical chemistry chemical physics : PCCP Vol. 22; no. 24; pp. 13358 - 13362 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
24.06.2020
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Online Access | Get full text |
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Summary: | Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells.
In cell
spin labeling using alkyne-azide click chemistry with a Gd
3+
-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.
A newly synthesized Gd
3+
chelate complex allows
in cell
spin labeling and detection of eGFP by EPR spectroscopy. |
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Bibliography: | 10.1039/d0cp01930e Electronic supplementary information (ESI) available. See DOI |
ISSN: | 1463-9076 1463-9084 |
DOI: | 10.1039/d0cp01930e |