Aptamer-enhanced fluorescence determination of bisphenol A after magnetic solid-phase extraction using FeO@SiO@aptamer

• Published in: Analytical methods Vol. 12; no. 36; pp. 4479 - 4486
• Main Authors: Xu, Zhou, Chen, Yanqiu, Tang, Yao, Chen, Maolong, Chen, Wei, Cheng, Yunhui
• Format: Journal Article
• Language: English
• Published: 24.09.2020
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/d0ay01124j

Bisphenol A (BPA) is used as a stabilizing agent in many food packaging plastics and is a known endocrine-disrupting chemical that can alter the development of mammary glands, affect egg cells, and cause chromosomal defects. However, the pretreatment of traditional assays for detecting BPA is difficult. In this work, a novel aptamer functionalized magnetic adsorbent was developed and combined with magnetic solid-phase extraction (MSPE) for the selective enrichment of BPA. First, magnetic silica-coated Fe 3 O 4 microspheres (Fe 3 O 4 @SiO 2 ) were synthesized by the sol-gel method, and functional magnetic nanoparticles (Fe 3 O 4 @SiO 2 @Apt) were formed by modifying with nucleic acids. In the presence of BPA in a MSPE system, the nucleic acid aptamer can specifically capture the target BPA. After magnetic separation, the Apt/BPA composite was eluted, and we observed enhanced fluorescence with the Apt/BPA composite that was formed. Our results showed that this method allowed a limit of detection of 0.05 ng mL −1 . The detection of bisphenol A based on the Fe 3 O 4 @SiO 2 @Aptamer nanoparticles.