Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d
Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readi...
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02.04.2019
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Abstract | Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to
in situ
imaging of miR-21 in different live cells.
DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively. |
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AbstractList | Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to
in situ
imaging of miR-21 in different live cells.
DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively. |
Author | Ding, Caifeng Li, Yuanfang Bi, Sai Song, Weiling Qi, Hongjie Yue, Shuzhen |
AuthorAffiliation | MOE Shandong Key Laboratory of Biochemical Analysis Qingdao University of Science and Technology Qingdao University College of Chemistry and Molecular Engineering College of Chemistry and Chemical Engineering Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science University-Industry Joint Center for Ocean Observation and Broadband Communication Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong |
AuthorAffiliation_xml | – name: MOE – name: Qingdao University of Science and Technology – name: Qingdao University – name: Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science – name: College of Chemistry and Chemical Engineering – name: University-Industry Joint Center for Ocean Observation and Broadband Communication – name: Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong – name: Shandong Key Laboratory of Biochemical Analysis – name: College of Chemistry and Molecular Engineering |
Author_xml | – sequence: 1 givenname: Yuanfang surname: Li fullname: Li, Yuanfang – sequence: 2 givenname: Shuzhen surname: Yue fullname: Yue, Shuzhen – sequence: 3 givenname: Hongjie surname: Qi fullname: Qi, Hongjie – sequence: 4 givenname: Caifeng surname: Ding fullname: Ding, Caifeng – sequence: 5 givenname: Weiling surname: Song fullname: Song, Weiling – sequence: 6 givenname: Sai surname: Bi fullname: Bi, Sai |
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Notes | imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI 10.1039/c9cc00747d in situ Electronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for |
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Snippet | Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize... |
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Title | Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d |
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