Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d

Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readi...

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Main Authors Li, Yuanfang, Yue, Shuzhen, Qi, Hongjie, Ding, Caifeng, Song, Weiling, Bi, Sai
Format Journal Article
Published 02.04.2019
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Abstract Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to in situ imaging of miR-21 in different live cells. DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively.
AbstractList Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to in situ imaging of miR-21 in different live cells. DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively.
Author Ding, Caifeng
Li, Yuanfang
Bi, Sai
Song, Weiling
Qi, Hongjie
Yue, Shuzhen
AuthorAffiliation MOE
Shandong Key Laboratory of Biochemical Analysis
Qingdao University of Science and Technology
Qingdao University
College of Chemistry and Molecular Engineering
College of Chemistry and Chemical Engineering
Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science
University-Industry Joint Center for Ocean Observation and Broadband Communication
Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong
AuthorAffiliation_xml – name: MOE
– name: Qingdao University of Science and Technology
– name: Qingdao University
– name: Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science
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– name: University-Industry Joint Center for Ocean Observation and Broadband Communication
– name: Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong
– name: Shandong Key Laboratory of Biochemical Analysis
– name: College of Chemistry and Molecular Engineering
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  givenname: Yuanfang
  surname: Li
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  surname: Song
  fullname: Song, Weiling
– sequence: 6
  givenname: Sai
  surname: Bi
  fullname: Bi, Sai
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Notes imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI
10.1039/c9cc00747d
in situ
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Title Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d
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