Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d
Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readi...
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Main Authors | , , , , , |
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Format | Journal Article |
Published |
02.04.2019
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Online Access | Get full text |
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Summary: | Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to
in situ
imaging of miR-21 in different live cells.
DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively. |
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Bibliography: | imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI 10.1039/c9cc00747d in situ Electronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for |
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/c9cc00747d |