Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imagingElectronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for in situ imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI: 10.1039/c9cc00747d

Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readi...

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Bibliographic Details
Main Authors Li, Yuanfang, Yue, Shuzhen, Qi, Hongjie, Ding, Caifeng, Song, Weiling, Bi, Sai
Format Journal Article
Published 02.04.2019
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Summary:Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to in situ imaging of miR-21 in different live cells. DNA nanobrushes are constructed based on target-triggered dynamic hairpin assembly in both unidirectional and bilateral growth manners, and realize sensitive and selective detection of short miRNA and long target DNA, respectively.
Bibliography:imaging of intracellular miRNAs, and the bilateral growth mechanism. See DOI
10.1039/c9cc00747d
in situ
Electronic supplementary information (ESI) available: Experimental section, non-denaturing PAGE characterization of unidirectional DHA, results of traditional CHA, DHA for
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc00747d