A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c9cc00261h

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleopr...

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Bibliographic Details
Main Authors Li, Jie, Røise, Joachim Justad, Zhang, Jingtuo, Yang, Jiaying, Kerr, D. Lucas, Han, Hesong, Murthy, Niren
Format Journal Article
LanguageEnglish
Published 11.04.2019
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Summary:In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption. A novel fluorescent single tailed surfactant was synthesized, which can disrupt endosomes, complex lipofectamine, and identify transfected cells.
Bibliography:10.1039/c9cc00261h
Electronic supplementary information (ESI) available. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc00261h