A microfluidic organotypic device for culture of mammalian intestines
The physiological characteristics of the gastrointestinal (GI) tract are diverse and include rapid rates of epithelial turnover, complex nervous and immune systems, a thick mucus layer, and a large microbial population. Most GI models in vitro rely upon cell lines or organoids and consequently lack...
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Published in | Analytical methods Vol. 12; no. 3; pp. 297 - 33 |
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Main Authors | , , , , , |
Format | Journal Article |
Published |
23.01.2020
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Online Access | Get full text |
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Summary: | The physiological characteristics of the gastrointestinal (GI) tract are diverse and include rapid rates of epithelial turnover, complex nervous and immune systems, a thick mucus layer, and a large microbial population. Most GI models
in vitro
rely upon cell lines or organoids and consequently lack the diversity of cells and microorganisms present
in vivo
.
In vivo
studies retain function and cellular diversity but are more difficult to control. Microfluidic tissue-on-a-chip devices provide powerful alternatives for modeling physiological systems. Such devices show promise for use in GI research; however, most models use non-physiologic culture environments with higher than
in vivo
oxygen levels and insufficient gut microbiota. Our goal is to create a bridge between
in vitro
and
in vivo
using microfluidic devices by incorporating
ex vivo
tissue explants in physiologically relevant environments. Here, we report a microfluidic organotypic device (MOD) that enables media flow with differential oxygen concentrations across luminal and muscular surfaces of gut tissue
ex vivo
. Tissue was shown to be viable for 72 h and lowering oxygen concentration to a more physiologic level impacted bacterial populations.
A microfluidic organotypic device that maintains mouse colon explants for up to 72 h in a physiologically relevant environment is reported. The device is easy to assemble and maintains physiologically accurate oxygen concentrations across the tissue. |
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Bibliography: | 10.1039/c9ay02038a Electronic supplementary information (ESI) available. See DOI |
ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/c9ay02038a |