Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational designElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ob00376a

• Main Authors: Fan, Bo, Dong, Wenxin, Chen, Tianyi, Chu, Jianlin, He, Bingfang
• Format: Journal Article
• Language: English
• Published: 04.04.2018

The 62nd residue of glycosyltransferase UGT BL 1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH t...